Oxis International Inc.

323 Vintage Park Drive, Suite B

Foster City, CA  92202  USA

Tel:  650-212-2568

Toll free:  800-547-3686

FAX: 650-212-2569

e-mail: info@oxis.com

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Catalog Number: 21055

Immunoassay for Nitrotyrosine

**For research use only. Not for use in diagnostic procedures.**

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Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from Superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e.g. ?1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and SOD. This form of protein nitration has been shown to be involved in the pathology of several human conditions such as chronic myocardial dysfunction, respiratory distress syndrome, inflammatory bowel disease, lung injury, asthma, atherosclerosis, rheumatoid arthritis, chronic renal failure, ALS, septic shock and others. The Oxis Nitrotyrosine-EIA is a sandwich ELISA. Antigen captured by a solid phase monoclonal antibody is detected with a biotin-labeled goat polyclonal anti-nitrotyrosine. A streptavidin peroxidase conjugate then binds to the biotinylated antibody. The tetramethylbenzidine (TMB) substrate is added and the yellow product is measured at 450 nm. Demonstration of nitrotyrosine in biological samples is useful in determining oxidative stress level and specifically infers the presence of peroxynitrite or related nitrogen-centered oxidants. The Nitrotyrosine ELISA kit from Oxis provides a simple reproducible method of NT detection in tissues, plasma and urine.

Specimen Type(s):
Tissue, Plasma, Urine

LLD = 2Nm

2 X 96 well plate

12 Months from date of manufacture when stored as specified

Kit Contents:
Nitrotyrosine Antibody
Streptavidin Peroxidase
TMB Substrate
Substrate Buffer
Substrate Diluting Buffer
Wash Buffer
Stop Solution
Pre-coated Plates w/Frame

Assay Precision:
Intra Assay (%CV) 2.32
Inter Assay (%CV) 11.17